pEGFP-C3-SFV nsP3

LOCUS       pEGFP-C3-SFV nsP3               6176 bp DNA     circular SYN 16-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   1243235
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             613..1329
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1351..2799
                     /codon_start=1
                     /label=SFV nsP3 (human codon)
                     /translation="APSYRVKRADIATCTEAAVVNAANARGTVGDGVCRAVAKKWPSAF
                     KGAATPVGTIKTVMCGSYPVIHAVAPNFSATTEAEGDRELAAVYRAVAAEVNRLSLSSV
                     AIPLLSTGVFSGGRDRLQQSLNHLFTAMDATDADVTIYCRDKSWEKKIQEAIDMRTAVE
                     LLNDDVELTTDLVRVHPDSSLVGRKGYSTTDGSLYSYFEGTKFNQAAIDMAEILTLWPR
                     LQEANEQICLYALGETMDNIRSKCPVNDSDSSTPPRTVPCLCRYAMTAERIARLRSHQV
                     KSMVVCSSFPLPKYHVDGVQKVKCEKGLLFDPTVPSVVSPRKYAASTTDHSDRSLRGFD
                     LDWTTDSSSTASDTMSLPSLQSCDIDSIYEPMAPIVVTADVHPEPAGIADLAADVHPEP
                     ADHVDLENPIPPPRPKRAAYLASRAAERPVPAPRKPTPAPRTAFRNKLPLTFGDFDEHE
                     VDALASGITFGDFDDVLRLGRAGA"
     primer_bind     1874..1897
                     /label=Seq-C3-1207-F
     polyA_signal    2964..3085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3092..3547)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3574..3678
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3680..4037
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3888..4023
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4072..4866
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5098..5145
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      5474..6062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgagctcaag gctcctagtt atcgggtgaa gcgcgcggac
     1381 attgctactt gtaccgaagc cgccgtagtc aacgccgcca atgctcgcgg caccgtgggc
     1441 gatggagtct gccgggccgt cgcgaaaaag tggccgagtg cgttcaaagg cgcggcaacc
     1501 ccagttggga caattaagac cgtcatgtgt ggcagctatc cggtcatcca cgccgtcgcc
     1561 cctaacttct cagcaacaac cgaggctgaa ggggaccggg aactcgccgc ggtatataga
     1621 gcggtggcag ccgaggtaaa cagattgtcc ttgagttccg ttgcaattcc ccttcttagc
     1681 acaggtgtgt ttagcggtgg gcgcgacaga ctgcagcagt ccctgaacca tctctttact
     1741 gccatggatg cgacggatgc ggatgtcacc atctattgcc gcgataagag ttgggagaag
     1801 aagattcaag aggccattga tatgaggacc gcagtagagc tgctgaatga cgacgtagag
     1861 cttacgacag acctggtcag agttcatccc gacagttccc tcgttggaag aaagggttac
     1921 agcacgactg acggatcctt gtactcatac tttgaaggta ctaagttcaa ccaagctgcg
     1981 attgatatgg ccgaaatttt gacgttgtgg ccacgcctcc aagaggcgaa cgagcaaata
     2041 tgtctgtatg ctctcgggga gactatggac aacattcgaa gtaaatgccc agtcaacgat
     2101 tcagattcta gcactccccc taggactgtc ccctgcttgt gtaggtacgc catgacggcc
     2161 gagcgaatag ctcggcttcg ctctcatcaa gtcaaatcaa tggtggtatg ttcaagtttc
     2221 ccccttccga aataccatgt agacggggtc cagaaagtca agtgcgaaaa aggtcttttg
     2281 ttcgatccta ctgtaccctc tgtagtctct ccgaggaagt atgccgctag taccacagat
     2341 cactccgaca ggagtttgcg ggggttcgac cttgattgga ccactgactc ttcctcaacg
     2401 gctagtgata cgatgtcatt gccaagcctg caatcatgtg acattgacag tatctatgag
     2461 ccgatggctc ccattgtggt cacggctgat gtacatccag aacccgctgg aatcgcagac
     2521 cttgcggctg acgttcaccc cgaaccagca gatcacgttg acctggagaa tccgattcct
     2581 cctccgaggc ctaagagagc cgcgtacctg gcatcacggg cggctgaacg gcccgtccct
     2641 gcgccaagga aacccactcc cgccccacgc actgcattta gaaataagct ccccctgact
     2701 ttcggagatt tcgatgaaca tgaagtagac gcgctcgcta gtgggataac ttttggagat
     2761 tttgatgacg tcctccgact gggaagagct ggggcttagc ttcgaattct gcagtcgacg
     2821 gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc cataccacat
     2881 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2941 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3061 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     3121 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3181 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     3241 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3301 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3361 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3421 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3481 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3541 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3601 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3661 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     3721 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3781 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3841 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3901 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3961 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4021 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     4081 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4141 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4201 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     4261 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4321 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4381 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4441 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4501 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4561 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     4621 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4681 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4741 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4801 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4861 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4921 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4981 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     5041 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     5101 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     5161 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     5221 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     5281 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     5341 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5401 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5461 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5521 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5581 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5641 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5701 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5761 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5821 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5881 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5941 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6001 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6061 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//